Carbonic Anhydrase 14 Polyclonal Antibody

Catalogue Numbers: BS62322-50, BS62322-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9ULX7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q22, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13.3, 15q22 and 1q21.2, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas. Human CA XIV is also expressed in normal tissues, such as brain, but differs from CA XII in its expression pattern.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Carbonic Anhydrase 14 polyclonal antibody detects endogenous levels of Carbonic Anhydrase 14 protein.

Extra Notes: Western blot (WB) analysis of Carbonic Anhydrase 14 polyclonal antibody at 1:500 dilution
LaneA:CT26 whole cell lysate
LaneB:PC12 whole cell lysate
LaneC:MCF-7 whole cell lysate

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Carbonic anhydrase 14; Carbonate dehydratase XIV; Carbonic anhydrase XIV; CA-XIV; CA14

Immunogen: Synthetic peptide, corresponding to Human Carbonic Anhydrase 14.

Conjugate: Unconjugated

Modification: Unmodified