SmartCheck ladders are available in 3 size ranges – 50bp, 100bp and 1kb – to cover most common applications.  

• Ready-to-use formulation includes loading buffer and tracking dye
• 500µl suitable for 100 lanes (5µl per lane)
• Higher intensity reference bands
• Ultra pure production allows economical ambient shipping

Technical Details

Protocol:  Briefly vortex the tube and use 5µl per lane. Additional loading buffer is not required.

Concentration:  0.1 mg/ml

Tracking dye: bromophenol blue and xylene cyanol

Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol

Shipping and Storage:  SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.

Production:  SmartCheck™ ladders are produced from proprietary plasmids, digested to completion.  A multistep chromatography method is used to ensure purity and DNA quality.

Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.