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10L Media Kit: MES-Volvox Medium10L Media Kit: MES Volvox Medium General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modification of Volvox Medium. MES buffer is used in place of glycylglycine, and ammonium chloride is added as a second nitrogen source. Media kit includes all the necessary chemical components to prepare 10 Liters of MES Volvox Medium. All UTEX Media Kit solutions are shipped sterile and should

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10L Media Kit: MES-Volvox Medium

Name: 10L Media Kit: MES-Volvox Medium
Brand: geomorr.pl
SKU: 43244233954
Price: 170.00 USD
Availability: InStock
Rating: 4.837000000000001 (76 reviews)

General purpose medium for freshwater strains, especially those requiring ammonium. Suitable for xenic and axenic cultures. Modification of Volvox Medium. MES buffer is used in place of glycylglycine, and ammonium chloride is added as a second nitrogen source.

Media kit includes all the necessary chemical components to prepare 10 Liters of MES-Volvox Medium. All UTEX Media Kit solutions are shipped sterile and should be handled aseptically to avoid contamination.

What is a media kit? How is it different than stock solutions?

UTEX Media Kits come with pre-measured, ready-to-use components. Unlike stock solutions, there is no need to prepare and/or dilute concentrated reagents for each individual chemical at precise ratios. Simply follow the included instructions to create the large volume of media—precision without the hassle of extra preparation.

Do the media kits include stock solutions?

No. UTEX Media Kits do not include concentrated stock solutions for each individual chemical component of a medium. Compatible chemicals have been pre-measured and combined to not only make preparation easier, but also reduce packaging and shipping costs.

What if I want individual stock solutions?

Each culture medium page includes a recipe that, when applicable, provides instructions for preparing stock solutions and how much to add. Because some components aren’t suitable for stock solutions, related instructions are provided only when applicable.

Looking for MES-Volvox Medium in 1-Liter and test tube volumes?

View/purchase prepared and sterile 1X MES-Volvox Medium

Medium At A Glance
Water Type	Freshwater
pH of Medium	6<pH<8
Contains	Micronutrients, NH₄, NO₃, Biotin, Vitamin B₁₂

Directions

For 10L Total Volume

Obtain and wear the appropriate PPE.
To approximately 9.6 Liters (9,600 mL) of dH₂O, add the first three solutions (Components #1-3) in order while stirring.
Bring total volume to 9.9 Liters (9,900 mL) with dH₂O.
Cover and autoclave or filter-sterilize if non-sterile water was used.
When cooled, aseptically add Component #4.
Store at refrigerator temperatures.

Note: When combined this gives a pH of 6.5 before autoclaving. Both the standard medium and medium made from the 10L Media Kit show a pH of 6.5 - 6.6 after autoclaving.

Chemical	Final Concentration
Ca(NO₃)₂•4H₂O	0.5 mM
MgSO₄•7H₂O	0.16 mM
Na₂glycerophosphate•5H₂O	0.16 mM
KCl	0.67 mM
NH₄Cl	0.5 mM
MES	10 mM
P-IV Metal Solution
Vitamin B₁₂
Biotin Vitamin Solution