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F/2 MediumF 2 Medium Directions For 1 Liter Total Volume To approximately 950 mL of non pasteurized seawater (30 ppt), add each of the components in the order specified (except vitamins) while stirring continuously. Bring total volume to 1 Liter with non pasteurized seawater (30 ppt). For 1. 5% (w v) agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium or filter sterilize. When cooled add sterile vitamins. For agar medium add
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F/2 Medium
Directions
For 1 Liter Total Volume
- To approximately 950 mL of non-pasteurized seawater (30 ppt), add each of the components in the order specified (except vitamins) while stirring continuously.
- Bring total volume to 1 Liter with non-pasteurized seawater (30 ppt).
- For 1.5% (w/v) agar medium add 15 g of agar into the flask; do not mix.
- Cover and autoclave medium or filter-sterilize.
- When cooled add sterile vitamins.
- For agar medium add vitamins, mix, and dispense before agar solidifies.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | NaNO3 (Fisher BP360-500) |
1 mL | 7.5 g/100 mL d-H2O | 880 µM |
| 2 | NaH2PO4•H2O (MCIB 742) |
1 mL | 0.5 g/100 mL d-H2O | 36 µM |
| 3 | Na2SiO3•9H2O (Sigma 307815) |
1 mL | 3 g/100 mL d-H2O | 106 µM |
| 4 | Trace Metals Solution | 1 mL/L | ||
| 5 | Vitamin B12 | 1 mL/L | ||
| 6 | Biotin Vitamin Solution | 1 mL/L | ||
| 7 | Thiamine Vitamin Solution | 1 mL/L |
F/2 Medium
Item no
:
61942361937
US$ 60.00
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Ships within 48 hours · Estimated delivery Jun 20 - Jun 25
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- Free Standard Shipping on $100+ Orders to the USA.
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