Rapid and simple detection system of apoptotic cells, with simultaneous differentiation of necrotic cells.

During the first stages of apoptosis, phosphatidylserine (PS) is translocated from the inner membran layer to the outer surface of the cell. The ROTITEST^®Annexin V Kit uses the Ca²⁺ dependent binding efficiency of Annexin V to PS in order to label cells with damaged cell membranes. Additionally, propidium iodide is used to counter stain nuclei of cells with opened membranes, hence those which undergo necrotic degradation.Thus, cells with yellow-green membrane staining only can be identified as apoptotic, while double stained cells (with yellow-green membranes plus orange-red nuclei) are classified as necrotic. Analysis is performed via flow cytometry or fluorescence microscopy.

Each package contains simple-to-follow instructions-for-use.

Annexin V-FITC conjugate, propidium iodide solution, Annexin V binding buffer, detailed instructions-for-use.Excluding the columns, contents of this Kit may not be bought separately.